Development of a mitochondrial DNA real‐time polymerase chain reaction assay for quality control of pathogen reduction with riboflavin and ultraviolet light
Abstract
Transfusion is associated with a risk of infection and alloimmunization. Pathogen reduction using riboflavin and UV light (Mirasol treatment) inactivates pathogens and leucocytes. With increasing adoption of the technology in clinical use, regulatory agencies have recommended the introduction of quality control measures to monitor pathogen reduction efficacy. We sought to develop a real‐time PCR‐based assay to document the impact of pathogen reduction on the mitochondrial genome in blood components.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 27, 2014
- Source ID
- 10.1111/vox.12173
Entities
People
- D. M. Chafets
- J. M. Mundt
- L. Wen
- M. P. Busch
- P. F. Van Der Meer
- R. P. Goodrich
- S. Bakkour
- S. Marschner
- T.‐h. Lee
Organizations
- Blood Systems Research Institute
- Terumo BCT (United States)
- United States Army