Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns
Abstract
Existing clinical approaches and tools to measure burn tissue destruction are limited resulting in misdiagnosis of injury depth in over 40% of cases. Thus, our objective in this study was to characterize the ability of short‐wave infrared (SWIR) imaging to detect moisture levels as a surrogate for tissue viability with resolution to differentiate between burns of various depths. To accomplish our aim, we constructed an imaging system consisting of a broad‐band Tungsten light source; 1,200‐, 1,650‐, 1,940‐, and 2,250‐nm wavelength filters; and a specialized SWIR camera. We initially used agar slabs to provide a baseline spectrum for SWIR light imaging and demonstrated the differential absorbance at the multiple wavelengths, with 1,940 nm being the highest absorbed wavelength. These spectral bands were then demonstrated to detect levels of moisture in inorganic and in vivo mice models. The multiwavelength SWIR imaging approach was used to diagnose depth of burns using an in vivo porcine burn model. Healthy and injured skin regions were imaged 72 hours after short (20 seconds) and long (60 seconds) burn application, and biopsies were extracted from those regions for histologic analysis. Burn depth analysis based on collagen coagulation histology confirmed the formation of superficial and deep burns. SWIR multispectral reflectance imaging showed enhanced intensity levels in long burned regions, which correlated with histology and distinguished between superficial and deep burns. This SWIR imaging method represents a novel, real‐time method to objectively distinguishing superficial from deep burns.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 04, 2019
- Source ID
- 10.1111/wrr.12779
Entities
People
- Benjamin Levi
- Charles Hwang
- David Cholok
- Jean Nemzek
- John Li
- Jonathan T. Butts
- Kavitha Ranganathan
- Mark Hemmila
- Michael D. Morris
- Omer Berenfeld
- Paul S Cederna
- Sergey Mironov
- Stewart C. Wang
- Vladislav A. Dolgachev
Organizations
- Abbott Laboratories
- American College of Surgeons
- International FOP Association
- Medtronic plc
- National Heart, Lung, and Blood Institute
- National Institute of General Medical Sciences
- United States Department of Defense
- University of Michigan