A cross‐species validation of single‐beat metrics of cardiac contractility
Abstract
The assessment of left ventricular (LV) contractility in animal models is useful in various experimental paradigms, yet obtaining such measures is inherently challenging and surgically invasive. In a cross‐species study using small and large animals, we comprehensively tested the agreement and validity of multiple single‐beat surrogate metrics of LV contractility against the field‐standard metrics derived from inferior vena cava occlusion (IVCO). Fifty‐six rats, 27 minipigs and 11 conscious dogs underwent LV and arterial catheterization and were assessed for a range of single‐beat metrics of LV contractility. All single‐beat metrics were tested for the various underlying assumptions required to be considered a valid metric of cardiac contractility, including load‐independency, sensitivity to inotropic stimulation, and ability to diagnose contractile dysfunction in cardiac disease. Of all examined single‐beat metrics, only LV maximal pressure normalized to end‐diastolic volume (EDV), end‐systolic pressure normalized to EDV, and the maximal rate of rise of the LV pressure normalized to EDV showed a moderate‐to‐excellent agreement with their IVCO‐derived reference measure and met all the underlying assumptions required to be considered as a valid cardiac contractile metric in both rodents and large‐animal models. Our findings demonstrate that single‐beat metrics can be used as a valid, reliable method to quantify cardiac contractile function in basic/preclinical experiments utilizing small‐ and large‐animal models image
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 05, 2022
- Source ID
- 10.1113/jp283319
Entities
People
- Alexandra M Williams
- Brian K Kwon
- Christopher R West
- Donal S. O'leary
- Erin Erskine
- Femke Streijger
- Filip Konečný
- Glen E Foster
- Javier A. Sala‐mercado
- Jennifer Duffy
- Joseph Mannozzi
- Katelyn Shortt
- Keerit Tauh
- Kitty So
- Kyoung-Tae Kim
- Liisa Wainman
- Mehdi Ahmadian
- Neda Manouchehri
- Ryan L. Hoiland
- Shera Fisk
Organizations
- Kyungpook National University
- MaRS Discovery District
- National Heart, Lung, and Blood Institute
- Natural Sciences and Engineering Research Council
- United States Department of Defense
- University of British Columbia
- University of British Columbia Okanagan
- Wayne State University