Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

Abstract

Probing base editing outcomes leads to new C:G to T:A base editors with greater efficiency and product purity, and fewer indels.

Document Details

Document Type
Pub Defense Publication
Publication Date
Aug 04, 2017
Source ID
10.1126/sciadv.aao4774

Entities

People

  • Ahmed H Badran
  • Alexis Komor
  • Amanda L. Waterbury
  • David R. Liu
  • Kevin T. Zhao
  • Luke W. Koblan
  • Michael S Packer
  • Nicole M Gaudelli
  • Y. Bill Kim

Organizations

  • Broad Institute
  • Harvard University
  • Howard Hughes Medical Institute
  • National Institute of Biomedical Imaging and Bioengineering
  • National Institute of General Medical Sciences

Tags

Readers

  • Mathematics or Statistics
  • Molecular Genetics