Ringlike Structure of the Deinococcus radiodurans Genome: A Key to Radioresistance?

Abstract

The bacterium Deinococcus radiodurans survives ionizing irradiation and other DNA-damaging assaults at doses that are lethal to all other organisms. How D . radiodurans accurately reconstructs its genome from hundreds of radiation-generated fragments in the absence of an intact template is unknown. Here we show that the D. radiodurans genome assumes an unusual toroidal morphology that may contribute to its radioresistance. We propose that, because of restricted diffusion within the tightly packed and laterally ordered DNA toroids, radiation-generated free DNA ends are held together, which may facilitate template-independent yet error-free joining of DNA breaks.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jan 10, 2003
Source ID
10.1126/science.1077865

Entities

People

  • Abraham Minsky
  • Ajay K. Sharma
  • Eyal Shimoni
  • Joseph Englander
  • Kenneth W. Minton
  • Smadar Levin-zaidman

Organizations

  • Uniformed Services University of the Health Sciences
  • Weizmann Institute of Science

Tags

Fields of Study

  • Biology
  • Physics

Readers

  • Microbial Pathology
  • Molecular Genetics
  • Polymer Science and Technology