Structure and Receptor Specificity of the Hemagglutinin from an H5N1 Influenza Virus

Abstract

The hemagglutinin (HA) structure at 2.9 angstrom resolution, from a highly pathogenic Vietnamese H5N1 influenza virus, is more related to the 1918 and other human H1 HAs than to a 1997 duck H5 HA. Glycan microarray analysis of this Viet04 HA reveals an avian α2-3 sialic acid receptor binding preference. Introduction of mutations that can convert H1 serotype HAs to human α2-6 receptor specificity only enhanced or reduced affinity for avian-type receptors. However, mutations that can convert avian H2 and H3 HAs to human receptor specificity, when inserted onto the Viet04 H5 HA framework, permitted binding to a natural human α2-6 glycan, which suggests a path for this H5N1 virus to gain a foothold in the human population.

Document Details

Document Type
Pub Defense Publication
Publication Date
Apr 21, 2006
Source ID
10.1126/science.1124513

Entities

People

  • Ian A Wilson
  • James C. Paulson
  • James D. Stevens
  • Jeffery Taubenberger
  • Ola Blixt
  • Terrence M. Tumpey

Organizations

  • Armed Forces Institute of Pathology
  • Centers for Disease Control and Prevention
  • Consortium for Functional Glycomics
  • Scripps Research

Tags

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Military Science
  • Molecular and Cellular Biochemistry