Recombination initiation maps of individual human genomes
Abstract
DNA double-strand breaks (DSBs) are introduced in meiosis to initiate recombination and generate crossovers, the reciprocal exchanges of genetic material between parental chromosomes. Here, we present high-resolution maps of meiotic DSBs in individual human genomes. Comparing DSB maps between individuals shows that along with DNA binding by PRDM9, additional factors may dictate the efficiency of DSB formation. We find evidence for both GC-biased gene conversion and mutagenesis around meiotic DSB hotspots, while frequent colocalization of DSB hotspots with chromosome rearrangement breakpoints implicates the aberrant repair of meiotic DSBs in genomic disorders. Furthermore, our data indicate that DSB frequency is a major determinant of crossover rate. These maps provide new insights into the regulation of meiotic recombination and the impact of meiotic recombination on genome function.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 14, 2014
- Source ID
- 10.1126/science.1256442
Entities
People
- Fatima Smagulova
- Florencia Pratto
- Galina V. Petukhova
- Kevin Brick
- Pavel Khil
- R. Daniel Camerini-otero
Organizations
- National Institutes of Health
- Uniformed Services University of the Health Sciences