Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans
Abstract
A whole-genome restriction map of Deinococcus radiodurans , a radiation-resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases) was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after γ irradiation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 03, 1999
- Source ID
- 10.1126/science.285.5433.1558
Entities
People
- Bhubaneswar Mishra
- Christopher Aston
- David C. Schwartz
- J. Craig Venter
- Jieyi Lin
- Junping Jing
- Kenneth W. Minton
- Michael J. Daly
- Owen White
- Rong Qi
- Thomas S. Anantharaman
Organizations
- Celera Corporation
- J. Craig Venter Institute
- New York University
- Uniformed Services University of the Health Sciences