Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation
Abstract
Polymyxins are antibiotics that disrupt the bacterial cell membrane and are used to treat multidrug-resistant infections. A bacterial enzyme called ArnT can mediate resistance to polymyxins by transferring a sugar group from a lipid carrier to lipid A, a component of the bacterial outer membrane. Petrou et al. described structures of ArnT alone and in complex with a lipid carrier and identified a cavity where lipid A probably binds. Insights into the enzyme mechanism could be exploited to design drugs that combat polymyxin resistance.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 05, 2016
- Source ID
- 10.1126/science.aad1172
Entities
People
- Brian Kloss
- Burkhard Rost
- Candice S. Klug
- Carmen M Herrera
- David Tomasek
- Edda Kloppmann
- Filippo Mancia
- Jérémie Vendome
- Kanagalaghatta R. Rajashankar
- Kathryn M. Schultz
- Lawrence Shapiro
- M Stephen Trent
- Meagan Belcher Dufrisne
- Oliver B. Clarke
- Surajit Banerjee
- Vasileios I. Petrou
Organizations
- Army Research Office
- Columbia University
- Cornell University
- Medical College of Wisconsin
- National Institute of General Medical Sciences
- National Institutes of Health
- Technical University of Munich
- University of Georgia