Single-molecule decoding of combinatorially modified nucleosomes
Abstract
Regulatory information is stored both in DNA sequences and in the chromatin proteins that package the genome. The covalent modification of histones plays a critical role in signaling whether a gene or genomic region should be active or inactive. Shema et al. used high-throughput single-molecule imaging to reveal the different combinations of modifications on millions of individual nucleosomes. Single-molecule DNA sequencing determined the exact location of these modified nucleosomes in the genome.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 06, 2016
- Source ID
- 10.1126/science.aad7701
Entities
People
- Bradley Bernstein
- Daniel R. Jones
- Efrat Shema
- Laura Donohue
- Noam Shoresh
- Ram Oren
Organizations
- Broad Institute
- Harvard Medical School
- Howard Hughes Medical Institute
- Jane Coffin Childs Memorial Fund for Medical Research
- National Human Genome Research Institute