Synthetic recombinase-based state machines in living cells
Abstract
Finite state machines are logic circuits with a predetermined sequence of actions that are triggered depending on the starting conditions. They are used for a variety of devices and biological systems, from vending machines to neural circuits. Roquet et al. have taken a finite state machine approach to control the expression of integrases, or enzymes that insert or excise phage DNA into or out of bacterial chromosomes. The integrases altered the DNA sequence of a plasmid to record all five possible combinations of two inputs. Such circuits can be used to record the states that the cell experienced over time and can be deployed in state-dependent gene expression programs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 22, 2016
- Source ID
- 10.1126/science.aad8559
Entities
People
- Alyssa C. Ferris
- Ava P. Soleimany
- Nathaniel Roquet
- Scott Aaronson
- Timothy K. Lu
Organizations
- Defense Advanced Research Projects Agency
- Ford Foundation
- Harvard University
- Massachusetts Institute of Technology
- National Institutes of Health
- National Science Foundation
- Office of Naval Research
- Wellesley College