Accurate design of megadalton-scale two-component icosahedral protein complexes
Abstract
Symmetric macromolecular structures that form cages, such as viral capsids, have inspired protein engineering. Bale et al. used pairwise combinations of dimeric, trimeric, or pentameric building blocks to design two-component, 120-subunit protein complexes with three distinct icosahedral architectures. The capsid-like nanostructures are large enough to hold nucleic acids or other proteins, and because they have two components, the assembly of cargoes such as drugs and vaccines can be done in a controlled way.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 22, 2016
- Source ID
- 10.1126/science.aaf8818
Entities
People
- Chantz Thomas
- Daniel Ellis
- David Baker
- Duilio Cascio
- Jacob B. Bale
- Neil P. King
- Shane Gonen
- Tamir Gonen
- Todd O. Yeates
- William Sheffler
- Yuxi Liu
Organizations
- Air Force Office of Scientific Research
- Defense Advanced Research Projects Agency
- Gates Foundation
- Howard Hughes Medical Institute
- Janelia Research Campus
- National Science Foundation
- Takeda Pharmaceutical Company
- University of California, Los Angeles
- University of Washington