Spatiotemporal microbial evolution on antibiotic landscapes
Abstract
A key aspect of bacterial survival is the ability to evolve while migrating across spatially varying environmental challenges. Laboratory experiments, however, often study evolution in well-mixed systems. Here, we introduce an experimental device, the microbial evolution and growth arena (MEGA)–plate, in which bacteria spread and evolved on a large antibiotic landscape (120 × 60 centimeters) that allowed visual observation of mutation and selection in a migrating bacterial front. While resistance increased consistently, multiple coexisting lineages diversified both phenotypically and genotypically. Analyzing mutants at and behind the propagating front, we found that evolution is not always led by the most resistant mutants; highly resistant mutants may be trapped behind more sensitive lineages. The MEGA-plate provides a versatile platform for studying microbial adaption and directly visualizing evolutionary dynamics.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 09, 2016
- Source ID
- 10.1126/science.aag0822
Entities
People
- Eric D. Kelsic
- Idan Yelin
- Michael Baym
- Remy Chait
- Rotem Gross
- Roy Kishony
- Tami D. Lieberman
Organizations
- European Research Council
- Harvard Medical School
- National Institutes of Health