Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes
Abstract
A new technique allows rapid purification of lysosomes and metabolic profiling by liquid chromatography and mass spectrometry. Abu-Remaileh et al. engineered cultured human cells to produce a protein tag on lysosomal membranes that could be used to rapidly precipitate purified lysosomes on magnetic beads. Analysis of their contents under various conditions showed that efflux from the lysosome of most essential amino acids (but not that of most other amino acids) is a regulated process. Amino acid transport was inhibited under conditions of nutrient depletion as a result of inhibition of the mTOR (mechanistic target of rapamycin) protein kinase complex.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 10, 2017
- Source ID
- 10.1126/science.aan6298
Entities
People
- Choah Kim
- David M. Sabatini
- Elizaveta Freinkman
- Gregory A Wyant
- Maria Abbasi
- Monther Abu-Remaileh
- Nouf N Laqtom
- Sze Ham Chan
Organizations
- Koch Institute for Integrative Cancer Research at MIT
- Massachusetts Institute of Technology
- National Institutes of Health
- United States Department of Defense