Multiplex recording of cellular events over time on CRISPR biological tape
Abstract
The CRISPR adaptation system has been used to record the sequence and ordering of exogenous oligonucleotides that are electroporated into cell populations. Sheth et al. engineered a system bypassing the use of exogenous DNA to directly record temporal signals. An input biological signal is transformed into the ratio of the frequency of incorporating trigger DNA to that of incorporating reference DNA into the genomes of a bacterial population. A multiplexing strategy enables simultaneous recording of three environmental signals with high temporal resolution.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 15, 2017
- Source ID
- 10.1126/science.aao0958
Entities
People
- Felix L. Wu
- Harris H. Wang
- Ravi U Sheth
- Sung Sun Yim
Organizations
- Alfred P. Sloan Foundation
- Columbia University
- Hertz Foundation
- National Institutes of Health
- National Science Foundation
- Office of Naval Research