Cellular checkpoint control using programmable sequential logic
Abstract
Synthetic genetic and biological regulatory circuits can enable logic functions to form the basis of biological computing; synthetic biology can also be used to control cell behaviors (see the Perspective by Glass and Alon). Andrews et al. used mathematical models and computer algorithms to combine standardized components and build programmable genetic sequential logic circuits. Such circuits can perform regulatory functions much like the biological checkpoint circuits of living cells. Circuits composed of interacting proteins could be used to bypass gene regulation, interfacing directly with cellular pathways without genome modification. Gao et al. engineered proteases that regulate one another, respond to diverse inputs that include oncogene activation, process signals, and conditionally activate responses such as those leading to cell death. This platform should facilitate development of “smart” therapeutic circuits for future biomedical applications.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 21, 2018
- Source ID
- 10.1126/science.aap8987
Entities
People
- Alec A K Nielsen
- Christopher Voigt
- Lauren B Andrews
Organizations
- Broad Institute
- Massachusetts Institute of Technology
- Office of Naval Research