Field-deployable viral diagnostics using CRISPR-Cas13
Abstract
CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al. added a sample preparation protocol to create a field-deployable viral diagnostic platform for rapid detection of specific strains of pathogens in clinical samples. Cas12a (also known as Cpf1), a type V CRISPR protein, cleaves double-stranded DNA and has been adapted for genome editing. Chen et al. discovered that Cas12a also processes single-stranded DNA threading activity. A technology platform based on this activity detected human papillomavirus in patient samples with high sensitivity.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 27, 2018
- Source ID
- 10.1126/science.aas8836
Entities
People
- Adriano Mondini
- Amanda L. Tan
- Ann Fiegen Durbin
- Bridget Chak
- Bronwyn L. Macinnis
- Cameron Myhrvold
- Catherine A Freije
- Feng Zhang
- Hayden C Metsky
- Irene Bosch
- Ivette Lorenzana
- Jonathan S. Gootenberg
- Kayla G. Barnes
- Kimberly F Garcia
- Lauren M Paul
- Leda A Parham
- Lee Gehrke
- Maurício Lacerda Nogueira
- Max J Kellner
- Nathan L Yozwiak
- Omar O Abudayyeh
- Pardis C. Sabeti
- Scott F. Michael
- Sharon Isern
Organizations
- Faculdade de Medicina de São José do Rio Preto
- Florida Gulf Coast University
- Harvard Medical School
- Harvard T.H. Chan School of Public Health
- Harvard University
- Howard Hughes Medical Institute
- Icahn School of Medicine at Mount Sinai
- Massachusetts Institute of Technology
- Office of the Director
- São Paulo State University