Developmental barcoding of whole mouse via homing CRISPR
Abstract
A homing guide RNA (hgRNA) that directs CRISPR-Cas9 to its own DNA locus can diversify its sequence and act as an expressed genetic barcode. Kalhor et al. engineered a mouse line carrying 60 independent loci of hgRNAs, thus generating a large number of unique barcodes in various embryonic and extraembryonic tissues in fully developed mice. This method demonstrates lineage tracing from the very first branches of the development tree up to organogenesis events and was used to elucidate embryonic brain patterning.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 31, 2018
- Source ID
- 10.1126/science.aat9804
Entities
People
- Amanda Graveline
- George M. Church
- Kathleen Leeper
- Kian Kalhor
- Leo Mejia
- Prashant Mali
- Reza Kalhor
Organizations
- Harvard Medical School
- Harvard University
- Intelligence Advanced Research Projects Activity
- National Institutes of Health
- Sharif University of Technology
- University of California, San Diego