De novo design of self-assembling helical protein filaments
Abstract
There has been some success in designing stable peptide filaments; however, mimicking the reversible assembly of many natural protein filaments is challenging. Dynamic filaments usually comprise independently folded and asymmetric proteins and using such building blocks requires the design of multiple intermonomer interfaces. Shen et al. report the design of self-assembling helical filaments based on previously designed stable repeat proteins. The filaments are micron scale, and their diameter can be tuned by varying the number of repeats in the monomer. Anchor and capping units, built from monomers that lack an interaction interface, can be used to control assembly and disassembly.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 09, 2018
- Source ID
- 10.1126/science.aau3775
Entities
People
- Bradley Parry
- Christine Jacobs-wagner
- David Baker
- Eric M Lynch
- Gustav Oberdorfer
- Hao Shen
- James DeYoreo
- Jiajun Chen
- Jorge A Fallas
- Juan Jesus Vicente
- Justin Decarreau
- Justin M Kollman
- Lance Stewart
- Lei Wang
- Linda Wordeman
- Michael Wagenbach
- Nicholas Jannetty
- Quinton Dowling
- William Sheffler
Organizations
- Defense Advanced Research Projects Agency
- Howard Hughes Medical Institute
- Pacific Northwest National Laboratory
- Shandong University
- University of Washington
- Yale School of Medicine
- Yale University