Systematic discovery of natural CRISPR-Cas12a inhibitors
Abstract
Bacteria and their phages continually coevolve in a molecular arms race. For example, phages use anti-CRISPR proteins to inhibit the bacterial type I and II CRISPR systems (see the Perspective by Koonin and Makarova). Watters et al. and Marino et al. used bioinformatic and experimental approaches to identify inhibitors of type V CRISPR-Cas12a. Cas12a has been successfully engineered for gene editing and nucleic acid detection. Some of the anti-Cas12a proteins identified in these studies had broad-spectrum inhibitory effects on Cas12a orthologs and could block Cas12a-mediated genome editing in human cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 12, 2018
- Source ID
- 10.1126/science.aau5138
Entities
People
- Christof Fellmann
- Hua B. Bai
- Jennifer Doudna
- Kyle E Watters
- Shawn M. Ren
Organizations
- Gladstone Institutes
- Howard Hughes Medical Institute
- National Institute of General Medical Sciences
- Paul G. Allen Family Foundation
- University of California
- University of California, Berkeley
- Yusuf Hamied Department of Chemistry