Discovery of widespread type I and type V CRISPR-Cas inhibitors
Abstract
Bacteria and their phages continually coevolve in a molecular arms race. For example, phages use anti-CRISPR proteins to inhibit the bacterial type I and II CRISPR systems (see the Perspective by Koonin and Makarova). Watters et al. and Marino et al. used bioinformatic and experimental approaches to identify inhibitors of type V CRISPR-Cas12a. Cas12a has been successfully engineered for gene editing and nucleic acid detection. Some of the anti-Cas12a proteins identified in these studies had broad-spectrum inhibitory effects on Cas12a orthologs and could block Cas12a-mediated genome editing in human cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 12, 2018
- Source ID
- 10.1126/science.aau5174
Entities
People
- Adair L Borges
- Alexander A. Sousa
- Benjamin J. Rauch
- Benjamin P Kleinstiver
- J. Keith Joung
- Jenny Y Zhang
- Joel D. Berry
- Joseph Bondy-Denomy
- Lina M. Leon
- Nicole D Marino
- Russell T Walton
Organizations
- Defense Advanced Research Projects Agency
- Harvard Medical School
- Massachusetts General Hospital
- National Institutes of Health
- University of California, San Francisco