Complex signal processing in synthetic gene circuits using cooperative regulatory assemblies
Abstract
Synthetic biologists would like to be able to make gene regulatory circuits that mimic key properties of eukaryotic gene regulation. Taking a cue from multimeric transcription factor complexes, Bashor et al. developed synthetic transcriptional circuits that produce nonlinear behavior from cooperativity (see the Perspective by Ng and El-Samad). Their system uses clamp proteins with multiple protein-interaction domains. Circuit behavior can be tuned by altering the number or affinities of the interactions according to a mathematical model. The authors created synthetic circuits with desired functions common in biology, for example, switch-like behavior or Boolean decision functions.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 10, 2019
- Source ID
- 10.1126/science.aau8287
Entities
People
- Ahmad S Khalil
- Ali Beyzavi
- Caleb J Bashor
- James J. Collins
- Jané Kondev
- Nikit Patel
- Sandeep Choubey
Organizations
- Boston University
- Brandeis University
- Broad Institute
- Defense Advanced Research Projects Agency
- Harvard University
- Massachusetts Institute of Technology
- National Institutes of Health
- National Science Foundation
- Rice University