Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution
Abstract
Optical and electron microscopy have made tremendous inroads into understanding the complexity of the brain. Gao et al. introduce an approach for high-resolution tracing of neurons, their subassemblies, and their molecular constituents over large volumes. They applied their method, which combines expansion microscopy and lattice light-sheet microscopy, to the mouse cortical column and the entire Drosophila brain. The approach can be performed at speeds that should enable high-throughput comparative studies of neural development, circuit stereotypy, and structural correlations to neural activity or behavior.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 18, 2019
- Source ID
- 10.1126/science.aau8302
Entities
People
- Adam Hantman
- Alfredo RodrÃguez
- Austin Graves
- C. Shan Xu
- Carolyn M. Ott
- Christopher Zugates
- Daniel E Milkie
- Edward Boyden
- Eric Betzig
- Gerald M. Rubin
- Grace H. Huynh
- Harald F. Hess
- Hilda Amalia Pasolli
- Igor Pisarev
- Jennifer Colonell
- Jennifer Lippincott-Schwartz
- John A. Bogovic
- Kishore R. Mosaliganti
- Ruixuan Gao
- Sean G. Megason
- Shoh M Asano
- Shu-Hsien Sheu
- Song Pang
- Srigokul Upadhyayula
- Stephan Saalfeld
- Susan Tappan
- Tomas Kirchhausen
- Tsung-li Liu
- Ved Singh
- Yongxin Zhao
- Yoshinori Aso
Organizations
- Boston Children's Hospital
- Harvard Medical School
- Helen Wills Neuroscience Institute
- Howard Hughes Medical Institute
- Intelligence Advanced Research Projects Activity
- MBF Bioscience
- Massachusetts Institute of Technology
- National Institutes of Health
- Open Philanthropy Project
- United States Army