Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
Abstract
Imaging the changes in fluorescence of voltage-sensitive reagents would enable monitoring of the activity of neurons in vivo. Abdelfattah et al. created such a voltage indicator by designing a protein that combines the voltage sensor domain from microbial rhodopsin with a domain that captures a dye molecule with exceptional brightness and photostability. When the protein was expressed in mice, flies, or zebrafish, they could monitor single action potentials in dozens of neurons simultaneously for many minutes.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 16, 2019
- Source ID
- 10.1126/science.aav6416
Entities
People
- Ahmed S Abdelfattah
- Amrita Singh
- Bei-jung Lin
- Brett D. Mensh
- Eric R Schreiter
- Gabe J. Murphy
- Glenn C Turner
- Hui Liu
- Jianing Yu
- Jihong Zheng
- Johannes Friedrich
- John J. Macklin
- Jonathan Grimm
- Karel Svoboda
- Kaspar Podgorski
- Liam Paninski
- Luke Campagnola
- Luke D. Lavis
- Minoru Koyama
- Misha B Ahrens
- Ondřej Novák
- Ronak Patel
- Stephanie C Seeman
- Takashi Kawashima
- Tsai-wen Chen
- Yi-Chieh Huang
- Yichun Shuai
- Zhe Liu
Organizations
- Allen Institute for Brain Science
- Columbia University
- Flatiron Institute
- Howard Hughes Medical Institute
- Johns Hopkins University
- National Science and Technology Council
- National Yang-Ming University
- Simons Foundation