Stem cell differentiation trajectories in Hydra resolved at single-cell resolution
Abstract
Hydra continually renews all cells in its body using three stem cell populations. This feature of Hydra allowed Siebert et al. to identify the transcriptional signatures of stem cells, progenitors, and terminally differentiated cells using single-cell RNA sequencing of adult Hydra (see the Perspective by Reddien). From these data, they built differentiation trajectories for all cell lineages, identified gene modules expressed along these trajectories, and identified putative regulators of genes within these modules. In addition, they identified candidate markers for elusive cell populations (such as multipotent stem cells and germline stem cells) and built a molecular map of the nervous system.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 26, 2019
- Source ID
- 10.1126/science.aav9314
Entities
People
- Abby S Primack
- Celina E Juliano
- Christine Schnitzler
- Jack F Cazet
- Jeffrey A Farrell
- Stefan Siebert
- Yashodara Abeykoon
Organizations
- Harvard University
- National Institutes of Health
- University of California
- University of California, Davis, College of Biological Sciences
- University of Florida