Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems
Abstract
Identifying where specific RNAs occur within a cell or tissue has been limited by technology and imaging capabilities. Expansion microscopy has allowed for better visualization of small structures by expanding the tissues with a polymer- and hydrogel-based system. Alon et al. combined expansion microscopy with long-read in situ RNA sequencing, resulting in a more precise visualization of the location of specific transcripts. This method, termed “ExSeq” for expansion sequencing, was used to detect RNAs, both new transcripts and those previously demonstrated to localize to neuronal dendrites. Unlike other in situ sequencing methods, ExSeq does not target sets of genes. This technology thus unites spatial resolution, multiplexing, and an unbiased approach to reveal insights into RNA localization and its physiological roles in developing and active tissue.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 29, 2021
- Source ID
- 10.1126/science.aax2656
Entities
People
- A. Bruna
- A. Dariush
- A. Emenari
- A. Fatemi
- A. J. Roth
- A. Küpcü Yoldaş
- A. Mcpherson
- A. Shea
- A. Sinha
- A. Smith
- A. T. Wassie
- Adam H. Marblestone
- Ana Lako
- Andrew C Payne
- Andrew G. Xue
- Anubhav Sinha
- Asmamaw T. Wassie
- Atsushi Kajita
- Aviv Regev
- B. Bodenmiller
- Bruce E. Johnson
- C. Caldas
- C. González-fernández
- C. H. O'flanagan
- C. Jauset
- C. M. Mulvey
- C. Xia
- Chih-Chieh Jay Yu
- Chun-chen Yao
- D. Bressan
- D. Lai
- Daniel Goodwin
- Daniel L. Abravanel
- E. A. González-solares
- E. D. Karagiannis
- E. Fisher
- E. Laks
- E. S. Boyden
- Ed S Lein
- Edward Boyden
- Eric B. Williams
- Eswar Prasad R. Iyer
- Evan R Daugharthy
- F. Grimaldi
- F. Nugent
- F. Qosaj
- Fei Chen
- G. Battistoni
- G. J. Hannon
- G. Lerda
- George M. Church
- H. Casbolt
- H. R. Ali
- Hanwool Lee
- Ho-jun Suk
- I. G. Cannell
- I. Pearsall
- I. Vázquez-garcía
- Imaxt Consortium
- J. A. Joyce
- J. Weselak
- J. Windhager
- Jeremy A. Miller
- Jialu Fan
- Johanna Klughammer
- Judit Jané‐Valbuena
- Julia Waldman
- K. Dinh
- K. Sawicka
- Karl Marrett
- Karla Helvie
- L. A. Sepúlveda
- L. Kuett
- M. Al Sa’d
- M. Callari
- M. Paez-ribes
- Marcel Burger
- Michal Slyper
- Minah Lee
- N. A. Walton
- N. Chornay
- N. Millar
- Nicole Cullen
- Nikhil Wagle
- Nikita Pak
- O. Harris
- O. M. Rueda
- Orit Rozenblatt-Rosen
- Paul L Reginato
- Paul Tillberg
- Peng Zheng
- R. Becker
- R. Kunes
- Richie E. Kohman
- Robert Prior
- Ru Wang
- S. A. Wild
- S. Aparicio
- S. Balasubramanian
- S. Harris
- S. L. Vogl
- S. S. Watson
- S. Tavaré
- S. Tietscher
- Sagar Shah
- Scott Rodig
- Shahar Alon
- Songlei Liu
- Sukanya Punthambaker
- T. Kovačević
- T. Ruiz
- T. Whitmarsh
- W. Greenwood
- Xinming Zhuang
- Y. Cui
- Y. Eyal-lubling
- Yang Li
- Yi Cui
- Yosuke Bando
Organizations
- Allen Institute for Brain Science
- Army Research Office
- Broad Institute
- Cancer Research UK
- Chan Zuckerberg Initiative
- Congress of Industrial Organizations
- Dana–Farber Cancer Institute
- Harvard Medical School
- Howard Hughes Medical Institute
- Institute of Nanotechnology
- Intelligence Advanced Research Projects Activity
- Kioxia
- Massachusetts Institute of Technology
- National Institutes of Health
- National Science Foundation
- Wyss Institute for Biologically Inspired Engineering