Pervasive functional translation of noncanonical human open reading frames
Abstract
Using mass spectrometry, ribosome profiling, and several CRISPR-based screens, Chen et al. identified hundreds of previously uncharacterized functional micropeptides in the human genome (see the Perspective by Wei and Guo). Protein translation outside of annotated open reading frames (ORFs) in messenger RNAs and within ORFs in long noncoding RNAs is pervasive. A functional screen using CRISPR-Cas9 with single-cell transcriptomics suggested critical roles for hundreds of micropeptides. Micropeptides encoded by multiple short, upstream ORFs form stable protein complexes with the downstream canonical proteins encoded on the same messenger RNAs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 06, 2020
- Source ID
- 10.1126/science.aay0262
Entities
People
- Alexander P. Fields
- Andreas-David Brunner
- Britt Adamson
- Daniel N Itzhak
- J. Zachery Cogan
- James K Nuñez
- Jason Y. Li
- Jin Chen
- Jonathan Weissman
- Manuel D Leonetti
- Matthias Mann
Organizations
- Chan Zuckerberg Initiative
- Howard Hughes Medical Institute
- Jane Coffin Childs Memorial Fund for Medical Research
- Max Planck Institute of Biochemistry
- Office of the Director
- University of California
- University of Copenhagen