De novo design of protein logic gates
Abstract
Signaling in cells can occur through protein-protein interactions. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a similar structure but where one module can be designed to interact specifically with another module. Using monomers and covalently connected monomers as inputs and encoding specificity through designed hydrogen-bond networks allowed the construction of two-input or three-input gates based on competitive binding. The modular control elements were used to regulate the association of elements of transcription machinery and split enzymes in vitro and in yeast cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 03, 2020
- Source ID
- 10.1126/science.aay2790
Entities
People
- Andrew C Hunt
- Basile I M Wicky
- Christie Ciarlo
- David Baker
- Florian Busch
- Galen Dods
- Hana El-Samad
- Hanna Liao
- Jocelynn Pearl
- John Stamatoyannopoulos
- Matthew S. Wilken
- Mengxuan Jia
- Michael C Jewett
- Ryan D Kibler
- Scott E Boyken
- Shon Green
- Vicki H. Wysocki
- Zachary L VanAernum
- Zibo Chen
Organizations
- Air Force Research Laboratory
- Altius Institute for Biomedical Sciences
- Army Research Office
- Burroughs Wellcome Fund
- Chan Zuckerberg Biohub
- David and Lucile Packard Foundation
- Defense Advanced Research Projects Agency
- European Molecular Biology Organization
- Howard Hughes Medical Institute
- National Institutes of Health
- Northwestern University
- Ohio State University
- Open Philanthropy Project
- University of California, San Francisco
- University of Luxembourg
- University of Washington