Designed proteins assemble antibodies into modular nanocages
Abstract
Antibodies are broadly used in therapies and as research tools because they can be generated against a wide range of targets. Efficacy can often be increased by clustering antibodies in multivalent assemblies. Divine et al. designed antibody nanocages from two components: One is an antibody-binding homo-oligomic protein and the other is the antibody itself. Computationally designed proteins drive the assembly of antibody nanocages in a range of architectures, allowing control of the symmetry and the antibody valency. The multivalent display enhances antibody-dependent signaling, and nanocages displaying antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein effectively neutralize pseudovirus.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 02, 2021
- Source ID
- 10.1126/science.abd9994
Entities
People
- Alex Roederer
- Alexandra Walls
- Ali Etemadi
- Andrew McGuire
- Daniel J Campbell
- David Baker
- David Veesler
- Franziska Seeger
- George Ueda
- Ha V Dang
- Hannele Ruohola-Baker
- Infencia Xavier Raj
- Ivan Vulovic
- James Lazarovits
- Jorge A Fallas
- Julie Mathieu
- Lance Stewart
- Leah J Homad
- Leonidas Stamatatos
- Madeleine F Jennewein
- Marti R. Tooley
- Mitchell L Fahning
- Mohammadali Mazloomi
- Neil P. King
- Peter A Morawski
- Robby Divine
- Shally Saini
- William Sheffler
- Yan Ting Zhao
- Yu-Hsin Wan
Organizations
- American Heart Association
- Benaroya Research Institute
- Fred Hutchinson Cancer Center
- Howard Hughes Medical Institute
- National Center for Advancing Translational Sciences
- National Institute of Allergy and Infectious Diseases
- National Institute of General Medical Sciences
- National Institutes of Health
- National Science Foundation
- Tehran University of Medical Sciences
- United States Department of Defense
- University of Washington
- Washington Research Foundation
- Wellcome