An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike
Abstract
Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously. By contrast, single-domain antibodies called nanobodies can be produced in bacteria or yeast, and their stability may enable aerosol delivery. Two papers now report nanobodies that bind tightly to spike and efficiently neutralize SARS-CoV-2 in cells. Schoof et al. screened a yeast surface display of synthetic nanobodies and Xiang et al. screened anti-spike nanobodies produced by a llama. Both groups identified highly potent nanobodies that lock the spike protein in an inactive conformation. Multivalent constructs of selected nanobodies achieved even more potent neutralization.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 18, 2020
- Source ID
- 10.1126/science.abe3255
Entities
People
- Aashish Manglik
- Adam Frost
- Aditya A Anand
- Adolfo García-Sastre
- Alexandrea N Rizo
- Alisa Bowen
- Almarie Joves
- Amber M Smith
- Amy Diallo
- Andrew W. Barile-hill
- Arceli Joves
- Arthur Melo
- Axel F Brilot
- Aye C Thwin
- Benjamin Barsi-rhyne
- Bryan Faust
- Caleigh M Azumaya
- Camille R Simoneau
- Carlos Nowotny
- Christian B Billesbølle
- Christian Bache Billesbølle
- Corie Y. Ralston
- Cristina Puchades
- Cynthia M Chio
- Daniel Asarnow
- Daniel R. Southworth
- Danielle L Swaney
- David A. Agard
- David P Bulkley
- Devan Diwanji
- Donovan Trinidad
- Eric Tse
- Erron W. Titus
- Fei Li
- Feng Wang
- Fengbo Zhou
- Frank Moss
- Gregory E Merz
- Henry C Nguyen
- Huong Q Tran
- Huong T. Kratochvil
- Iris D Young
- Ishan Deshpande
- James S Fraser
- Jenny Chen
- Jessica K Peters
- Jiahao Liang
- Jianhua Zhao
- Joana Paulino
- Julian R. Braxton
- Junrui Li
- Justin T Biel
- Kaihua Zhang
- Kaitlin Schaefer
- Kate Kim
- Kliment A. Verba
- Kris M White
- Kristoffer E Leon
- Kyle E. Lopez
- Liam Mckay
- Loan Doan
- Mali Safari
- Marcell Zimanyi
- Marco Vignuzzi
- Mariano Tabios
- Megan Lo
- Meghna Gupta
- Melanie Ott
- Melody G. Campbell
- Michael C Thompson
- Michael Schoof
- Michelle Moritz
- Miles Sasha Dickinson
- Ming Sun
- Mingliang Jin
- Morgane Boone
- Nadia Herrera
- Natalia Jura
- Natalie Whitis
- Nevan J. Krogan
- Nevan Krogan
- Nick Hoppe
- Niv Dobzinski
- Oren S Rosenberg
- Paul V. Thomas
- Peter Walter
- Phuong Nguyen
- Qcrg Structural Biology Consortium
- Raphael Trenker
- Reuben A Saunders
- Robert M. Stroud
- Sayan Gupta
- Sebastian Flores
- Sergei Pourmal
- Shoshana Zha
- Silke Nock
- Smriti Sangwan
- Stephanie Wankowicz
- Tanja Kortemme
- Thomas H Pospiech
- Thomas H. Pospiech Jr.
- Tristan W Owens
- Tsz Kin Martin Tsui
- Un Seng Chio
- Ursula Schulze-Gahmen
- Veronica Rezelj
- Victor L. Lam
- Vladislav Belyy
- Xi Liu
- Yang Li
- Yang Zhang
- Yanxin Liu
- Yen-li Li
- Yifan Cheng
- Yuwei Liu
- Zanlin Yu
Organizations
- Alfred Benzon Foundation
- Damon Runyon Cancer Research Foundation
- Freedom Together Foundation
- Gladstone Institutes
- Helen Hay Whitney Foundation
- Howard Hughes Medical Institute
- Icahn School of Medicine at Mount Sinai
- Lawrence Berkeley National Laboratory
- National Institute of Allergy and Infectious Diseases
- National Institute of Neurological Disorders and Stroke
- National Institutes of Health
- Roddenberry Foundation
- The Pew Charitable Trusts
- United States Department of Education
- University of California, San Francisco