Prevalence of Quinolone Resistance in Enterobacteriaceae from Sierra Leone and the Detection of qnrB Pseudogenes and Modified LexA Binding Sites

Abstract

A collection of 74 Enterobacteriaceae isolates found in Bo, Sierra Leone, were tested for quinolone antibiotic susceptibility and resistance mechanisms. The majority of isolates (62%) were resistant to quinolones, and 61% harbored chromosomal gyrA and/or parC mutations. Plasmid-mediated quinolone resistance genes were ubiquitous, with qnrB and aac(6′)-Ib-cr being the most prevalent. Mutated LexA binding sites were found in all qnrB1 genes, and truncated qnrB pseudogenes were found in the majority of Citrobacter isolates.

Document Details

Document Type
Pub Defense Publication
Publication Date
Nov 01, 2016
Source ID
10.1128/aac.01576-16

Entities

People

  • Chris R. Taitt
  • Daniel Chae
  • David A. Stenger
  • Gary J Vora
  • Michael G. Stockelman
  • Rashid Ansumana
  • Tomasz Leski
  • Umaru Bangura

Organizations

  • Defense Threat Reduction Agency
  • Njala University
  • Office of Naval Research
  • Thomas Jefferson High School for Science and Technology
  • United States Naval Research Laboratory

Tags

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Military and Counterinsurgency Studies.
  • Molecular Genetics