An Escherichia coli Nitrogen Starvation Response Is Important for Mutualistic Coexistence with Rhodopseudomonas palustris
Abstract
Microbial mutualistic cross-feeding interactions are ubiquitous and can drive important community functions. Engaging in cross-feeding undoubtedly affects the physiology and metabolism of individual species involved. However, the nature in which an individual species' physiology is influenced by cross-feeding and the importance of those physiological changes for the mutualism have received little attention. We previously developed a genetically tractable coculture to study bacterial mutualisms. The coculture consists of fermentative Escherichia coli and phototrophic Rhodopseudomonas palustris . In this coculture, E. coli anaerobically ferments sugars into excreted organic acids as a carbon source for R. palustris . In return, a genetically engineered R. palustris strain constitutively converts N 2 into NH 4 + , providing E. coli with essential nitrogen. Using transcriptome sequencing (RNA-seq) and proteomics, we identified transcript and protein levels that differ in each partner when grown in coculture versus monoculture. When in coculture with R. palustris , E. coli gene expression changes resembled a nitrogen starvation response under the control of the transcriptional regulator NtrC. By genetically disrupting E. coli NtrC, we determined that a nitrogen starvation response is important for a stable coexistence, especially at low R. palustris NH 4 + excretion levels. Destabilization of the nitrogen starvation regulatory network resulted in variable growth trends and, in some cases, extinction. Our results highlight that alternative physiological states can be important for survival within cooperative cross-feeding relationships.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 15, 2018
- Source ID
- 10.1128/aem.00404-18
Entities
People
- Alexandra L McCully
- D. Allan Drummond
- Evgeny V. Pilipenko
- James B McKinlay
- Jeffrey L. Mazny
- Jennifer R. Gliessman
- Megan G. Behringer
- Michael Lynch
Organizations
- Arizona State University
- Army Research Office
- Indiana University
- National Institutes of Health
- Office of Biological and Environmental Research
- University of Chicago