Secreted Flavin Cofactors for Anaerobic Respiration of Fumarate and Urocanate by Shewanella oneidensis: Cost and Role
Abstract
Shewanella species are prevalent in marine and aquatic environments, throughout stratified water columns, in mineral-rich sediments, and in association with multicellular marine and aquatic organisms. The diversity of niches shewanellae can occupy are due largely to their respiratory versatility. Shewanella oneidensis is a model organism for dissimilatory metal reduction and can respire a diverse array of organic and inorganic compounds, including dissolved and solid metal oxides. The fumarate reductase FccA is a highly abundant multifunctional periplasmic protein that acts to bridge the periplasm and temporarily store electrons in a variety of respiratory nodes, including metal, nitrate, and dimethyl sulfoxide respiration. However, maturation of this central protein, particularly flavin cofactor acquisition, is poorly understood. Here, we quantify the fitness cost of flavin secretion and describe how free flavins are acquired by FccA and a homologous periplasmic flavoprotein, UrdA.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 15, 2019
- Source ID
- 10.1128/aem.00852-19
Entities
People
- Augustus R. Pendleton
- Aunica L. Kane
- Catarina M Paquete
- Eric D. Kees
- Jeffrey A Gralnick
- Matthew B. Arriola
- Nicholas J. Kotloski
- Peter J. Intile
Organizations
- NOVA University Lisbon
- Office of Naval Research
- University of Minnesota