Response of Secondary Metabolism of Hypogean Actinobacterial Genera to Chemical and Biological Stimuli
Abstract
Microbial secondary metabolites are an important source of biologically active and therapeutically relevant small molecules. However, much of this active molecular diversity is challenging to access due to low production levels or difficulty in discerning secondary metabolites within complex microbial extracts prior to isolation. Here, we demonstrate that ecological stimuli increase secondary metabolite production in phylogenetically diverse actinobacteria isolated from understudied hypogean environments. Additionally, we show that comparative metabolomics linking stimuli to metabolite response data can effectively reveal secondary metabolites within complex biological extracts. This approach highlighted secondary metabolites in almost all observed natural product classes, including low-abundance analogs of biologically relevant metabolites, as well as a new linear aminopolyol polyketide, funisamine. This study demonstrates the generality of activating stimuli to potentiate secondary metabolite production across diverse actinobacterial genera.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 01, 2018
- Source ID
- 10.1128/aem.01125-18
Entities
People
- Audrey E. Ynigez-gutierrez
- Brett C. Covington
- Brian O. Bachmann
- Jeffrey M. Spraggins
- Zachary B. Hylton
Organizations
- National Institutes of Health
- National Science Foundation
- Vanderbilt University