Discovery and Characterization of NativeDeinococcus radioduransPromoters for Tunable Gene Expression
Abstract
The tolerance of the extremophile,Deinococcus radiodurans, to numerous oxidative stresses makes it ideal for bioremediation applications, but many of the tools necessary for metabolic engineering are lacking in this organism compared to model bacteria. Although native and engineered promoters have been used to drive gene expression for protein production inD. radiodurans, very few have been well characterized. Informed by bioinformatics, this study expands the repertoire of well-characterized promoters forD. radioduransvia thorough characterization of eight putative promoters with various strengths. These results will help facilitate tunable gene expression, since these promoters demonstrate strong and consistent performance compared to the current standard,PgroES. This study also provides a methodology for high-throughput promoter identification and characterization using fluorescence inD. radiodurans. The promoters identified in this study will facilitate metabolic engineering ofD. radioduransand enable its use in biotechnological applications ranging from bioremediation to synthesis of commodity chemicals.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 01, 2019
- Source ID
- 10.1128/aem.01356-19
Entities
People
- Angela Chen
- Cynthia Chu
- Lydia M Contreras
- Mark W. Sherman
- Natalia Gonzalez
- Tulshi Patel
Organizations
- Air Force Office of Scientific Research
- Defense Threat Reduction Agency
- National Science Foundation
- Robert A. Welch Foundation
- University of Texas at Austin