Repurposing CRISPR-Cas Systems as Genetic Tools for the Enterobacteriales
Abstract
Over the last decade, the study of CRISPR-Cas systems has progressed from a newly discovered bacterial defense mechanism to a diverse suite of genetic tools that have been applied across all domains of life. While the initial applications of CRISPR-Cas technology fulfilled a need to more precisely edit eukaryotic genomes, creative “repurposing” of this adaptive immune system has led to new approaches for genetic analysis of microorganisms, including improved gene editing, conditional gene regulation, plasmid curing and manipulation, and other novel uses.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 15, 2021
- Source ID
- 10.1128/ecosalplus.esp-0006-2020
Entities
People
- Gregory J. Phillips
- Nicholas Backes
Organizations
- Defense Threat Reduction Agency
- Iowa State University