Functional Properties of Helicobacter pylori VacA Toxin m1 and m2 Variants
Abstract
Helicobacter pylori colonizes the gastric mucosa and secretes a pore-forming toxin (VacA). Two main types of VacA, m1 and m2, can be distinguished by phylogenetic analysis. Type m1 forms of VacA have been extensively studied, but there has been relatively little study of m2 forms. In this study, we generated H. pylori strains producing chimeric proteins in which VacA m1 segments of a parental strain were replaced by corresponding m2 sequences. In comparison to the parental m1 VacA protein, a chimeric protein (designated m2/m1) containing m2 sequences in the N-terminal portion of the m region was less potent in causing vacuolation of HeLa cells, AGS gastric cells, and AZ-521 duodenal cells and had reduced capacity to cause membrane depolarization or death of AZ-521 cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 20, 2020
- Source ID
- 10.1128/iai.00032-20
Entities
People
- Anne M. Campbell
- Arwen E. Frick-cheng
- Diane Bimczok
- Johanna C. Sierra
- Keith T. Wilson
- Mandy D. Truelock
- Mark S. McClain
- Nora J. Foegeding
- Rhonda R. Caston
- Timothy L. Cover
Organizations
- Montana State University
- National Institutes of Health
- United States Department of Agriculture
- United States Department of Defense
- United States Department of Veterans Affairs
- Vanderbilt University