Initial Characterization of the Two ClpP Paralogs of Chlamydia trachomatis Suggests Unique Functionality for Each
Abstract
Chlamydia trachomatis is the leading cause of preventable infectious blindness and of bacterial sexually transmitted infections worldwide. Chlamydiae are developmentally regulated obligate intracellular pathogens that alternate between two functional and morphologic forms, with distinct repertoires of proteins. We hypothesize that protein degradation is a critical aspect to the developmental cycle. A key system involved in protein turnover in bacteria is the Clp protease system. Here, we characterized the two chlamydial ClpP paralogs by examining their expression in Chlamydia spp., their ability to oligomerize, and their proteolytic activity. This work will help understand the evolutionarily diverse Clp proteases in the context of intracellular organisms, which may aid in the study of other clinically relevant intracellular bacteria.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 15, 2019
- Source ID
- 10.1128/jb.00635-18
Entities
People
- Amanda M. Blocker
- Derek J Fisher
- Krystal Y. Chung
- Martin Conda-Sheridan
- Nathalia Rodrigues de Almeida
- Nicholas A. Wood
- Scot P. Ouellette
Organizations
- National Science Foundation
- Southern Illinois University
- United States Department of Defense
- University of Nebraska Medical Center
- University of Nebraska–Lincoln
- University of South Dakota Sanford School of Medicine