Impact of Early Antiretroviral Therapy on Detection of Cell-Associated HIV-1 Nucleic Acid in Blood by the Roche Cobas TaqMan Test

Abstract

The Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test, v2.0 (the CAP/CTM assay), was used to quantify cell-associated HIV-1 (CAH) nucleic acid in peripheral blood mononuclear cells (PBMC) from well-characterized clinical specimens from HIV-1-infected individuals on antiretroviral therapy (ART). Chronically infected individuals on ART with no detectable plasma HIV-1 RNA demonstrated average CAH burdens of 3.2 HIV-1 log 10 copies/million cells.

Document Details

Document Type
Pub Defense Publication
Publication Date
May 01, 2019
Source ID
10.1128/jcm.01922-18

Entities

People

  • Donn J. Colby
  • Eugène Kroon
  • Holly R. Hack
  • Jennifer A. Malia
  • Jintanat Ananworanich
  • Joanna Freeman
  • Linda L. Jagodzinski
  • Mark M. Manak
  • Mark de Souza
  • Michelle A. Lally
  • Nelson Michael
  • Nitiya Chomchey
  • Nittaya Phanuphak
  • Sheila A. Peel
  • Ying Liu

Organizations

  • Brown University
  • National Institutes of Health
  • Thai Red Cross Society
  • U.S. Military HIV Research Program
  • United States Army Medical Research and Development Command
  • Walter Reed Army Institute of Research

Tags

Fields of Study

  • Biology

Readers

  • Forest Ecology
  • Immunology

Technology Areas

  • Biotechnology