Comparative Performance of Five Commercially Available Serologic Assays To Detect Antibodies to SARS-CoV-2 and Identify Individuals with High Neutralizing Titers
Abstract
Accurate serological assays to detect antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to characterize the epidemiology of SARS-CoV-2 infection and identify potential candidates for coronavirus disease 2019 (COVID-19) convalescent plasma (CCP) donation. This study compared the performances of commercial enzyme immunoassays (EIAs) with respect to detection of IgG or total antibodies to SARS-CoV-2 and neutralizing antibodies (nAbs). The diagnostic accuracy of five commercially available EIAs (Abbott, Euroimmun, EDI, ImmunoDiagnostics, and Roche) for detection of IgG or total antibodies to SARS-CoV-2 was evaluated using cross-sectional samples from potential CCP donors who had prior molecular confirmation of SARS-CoV-2 infection ( n = 214) and samples from prepandemic emergency department patients without SARS-CoV-2 infection ( n = 1,099).
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 21, 2021
- Source ID
- 10.1128/jcm.02257-20
Entities
People
- Aaron Tobian
- Andrew D. Redd
- Andrew Pekosz
- Arturo Casadevall
- Charles S. Kirby
- David P Sullivan
- Denali Boon
- Eshan U Patel
- Estelle Piwowar-manning
- Ethan Klock
- Evan M Bloch
- Haley A. Schmidt
- Jernelle Miller
- Kirsten Littlefield
- Morgan Keruly
- Oliver Laeyendecker
- Owen R. Baker
- Philip Sullivan
- Reinaldo E. Fernandez
- Richard E. Rothman
- Ruchee Shrestha
- Shmuel Shoham
- Thomas C. Quinn
- William Clarke
- Yolanda Eby
- Yu-hsiang Hsieh
Organizations
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases
- Johns Hopkins Bloomberg School of Public Health
- Johns Hopkins University
- National Heart, Lung, and Blood Institute
- National Institute of Allergy and Infectious Diseases
- National Institute on Drug Abuse
- National Institutes of Health
- United States Department of Defense