Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing
Abstract
The long-lasting global COVID-19 pandemic demands timely genomic investigation of SARS-CoV-2 viruses. Here, we report a simple and efficient workflow for whole-genome sequencing utilizing one-step reverse transcription-PCR (RT-PCR) amplification on a microfluidic platform, followed by MiSeq amplicon sequencing.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 20, 2021
- Source ID
- 10.1128/jcm.02784-20
Entities
People
- Brett F. Beitzel
- Cynthia Y. Tang
- Detlef Ritter
- Hye Kyung Chung
- Irina Maljkovic Berry
- Jun Hang
- Mark A. Sanborn
- Papa K. Pireku
- Richard D. Hammer
- Tao Li
- Xiu-Feng Wan
Organizations
- University of Missouri
- Walter Reed Army Institute of Research