Dimerization of Dengue Virus E Subunits Impacts Antibody Function and Domain Focus
Abstract
Dengue virus vaccine development is particularly challenging because vaccines have to provide protection against four different dengue virus stereotypes. The leading dengue virus vaccine candidates in clinical testing are all based on live-virus vaccine platforms and struggle to induce balanced immunity. Envelope subunit antigens have the potential to overcome these limitations but have historically performed poorly as vaccine antigens, because the versions tested previously were presented as monomers and not in their natural dimer configuration. This study shows that the authentic presentation of DENV2 E-based subunits has a strong impact on antibody responses, underscoring the importance of mimicking the complex protein structures that are found on DENV particle surfaces when designing subunit vaccines.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 31, 2020
- Source ID
- 10.1128/jvi.00745-20
Entities
People
- Aravinda M. de Silva
- Ashlie Thomas
- Brian Kuhlman
- Devina Thiono
- John Forsberg
- Lakshmanane Premkumar
- Shaomin Tian
- Stefan W Metz
- Stephan T. Kudlacek
Organizations
- National Institutes of Health
- United States Department of Defense
- University of North Carolina