Modeling HIV-1 Latency Using Primary CD4+T Cells from Virally Suppressed HIV-1-Infected Individuals on Antiretroviral Therapy
Abstract
Primary cell models of HIV latency have been very useful to identify mechanisms contributing to HIV latency and to evaluate potential HIV cure strategies. However, the current models utilizein vitroinfection with exogenous virus that does not fully recapitulate virus reactivation profiles of endogenous HIV inin vivo-infected CD4+T cells. In contrast, obtaining sufficient amounts of CD4+T cells from HIV-infected individuals to interrogate the HIV reservoirin vitrorequires leukapheresis. In the model we propose here,in vitroexpansion and extended culture of primary CD4+T cells isolated from virally suppressed HIV-infected individuals enable obtaining large numbers of cells harboring endogenous latent HIV reservoirs without performing leukapheresis. This model captures the variability of HIV reservoirs seeded in different individuals and should be useful to evaluate future HIV cure strategies.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 01, 2019
- Source ID
- 10.1128/jvi.02248-18
Entities
People
- Aaron Sy
- Cari Kessing
- Hiroshi Takata
- Julia Sciumbata
- Luisa Mori
- Lydie Trautmann
- Nelson Michael
- Nicolas Chomont
- Noemia S Lima
- R. Brad Jones
- Susana Valente
Organizations
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- National Institute of Allergy and Infectious Diseases
- Scripps Research
- United States Department of Defense
- Université de Montréal
- Walter Reed Army Institute of Research
- Weill Cornell Medicine