Differential Effects of Influenza Virus NA, HA Head, and HA Stalk Antibodies on Peripheral Blood Leukocyte Gene Expression during Human Infection
Abstract
Influenza A viruses are significant human pathogens that caused 83,000 deaths in the United States during 2017 to 2018, and there is need to understand the molecular correlates of illness and to identify prognostic markers of viral infection, symptom severity, and disease course. Preexisting antibodies against viral neuraminidase (NA) and hemagglutinin (HA) proteins play a critical role in lessening disease severity. We performed global gene expression profiling of peripheral blood leukocytes collected during acute and convalescent phases from a large cohort of people infected with A/H1N1pdm virus. Using statistical and machine-learning approaches, populations of genes were identified early in infection that correlated with active viral shedding, predicted length of shedding, or disease severity. Finally, these gene expression responses were differentially affected by increased levels of preexisting influenza antibodies, which could mask detection of these markers of contagiousness and disease severity in people with active clinical disease.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 25, 2019
- Source ID
- 10.1128/mbio.00760-19
Entities
People
- Adriana Cervantes-medina
- Alison Han
- Colleen Bushell
- David Baxter
- Hao Wang
- Jae-keun Park
- Jeffery Taubenberger
- John C Kash
- Kathie-Anne Walters
- Kelsey Scherler
- Lindsay Czajkowski
- Loretta Auvil
- Luz Angela Rosas
- Matthew J. Memoli
- Michael Welge
- Min Young Lee
- Ruoqing Zhu
- Tyler Bristol
- Zainab Rahil
Organizations
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases
- Institute for Systems Biology
- National Center for Supercomputing Applications
- National Institute of Allergy and Infectious Diseases
- Statistics New Zealand