The Effects of Signal Erosion and Core Genome Reduction on the Identification of Diagnostic Markers

Abstract

Whole-genome sequence (WGS) data are commonly used to design diagnostic targets for the identification of bacterial pathogens. To do this effectively, genomics databases must be comprehensive to identify the strict core genome that is specific to the target pathogen. As additional genomes are analyzed, the core genome size is reduced and there is erosion of the target-specific regions due to commonality with related species, potentially resulting in the identification of false positives and/or false negatives.

Document Details

Document Type
Pub Defense Publication
Publication Date
Nov 02, 2016
Source ID
10.1128/mbio.00846-16

Entities

People

  • Adam J. Vazquez
  • Alex Hutcheson
  • Apichai Tuanyok
  • Bart J. Currie
  • Carina M. Hall
  • Christopher J. Allender
  • David M Wagner
  • Derek S Sarovich
  • Direk Limmathurotsakul
  • Erin P. Price
  • Galina Koroleva
  • Gumphol Wongsuwan
  • Gustavo Palacios
  • James M. Schupp
  • Jason T. Ladner
  • Jason W. Sahl
  • John J. Lipuma
  • Jonas Korlach
  • Joseph D. Busch
  • Kenzie Shippy
  • Madeline Lummis
  • Mark Mayo
  • Paul Keim
  • Rebecca E Colman
  • Sean Lovett
  • Talima Pearson
  • Vanaporn Wuthiekanun
  • Vanessa Theobald

Organizations

  • Charles Darwin University
  • Defense Threat Reduction Agency
  • Mahidol University
  • Northern Arizona University
  • Pacific Biosciences
  • Translational Genomics Research Institute
  • University of Florida
  • University of Michigan

Tags

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Sensor Fusion and Tracking Systems.
  • Systems Analysis and Design