The Symmetrical Wave Pattern of Base-Pair Substitution Rates across the Escherichia coli Chromosome Has Multiple Causes
Abstract
It has been found in several species of bacteria that the rate at which single base pairs are mutated is not constant across the genome but varies in a wave-like pattern that is symmetrical about the origin of replication. Using Escherichia coli as our model system, we show that this pattern is the result of several interconnected factors. First, the timing and progression of replication are important in determining the wave pattern. Second, the three-dimensional structure of the DNA is also a factor, and the results suggest that mutation rates increase when highly structured DNA is replicated. Finally, biases in error correction, which may be responsive both to the progression of DNA synthesis and to DNA structure, are major determinants of the wave pattern. These factors should apply to most bacterial and, possibly, eukaryotic genomes and suggest that different areas of the genome evolve at different rates.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 27, 2019
- Source ID
- 10.1128/mbio.01226-19
Entities
People
- Brittany A. Niccum
- Haixu Tang
- Heewook Lee
- Patricia L. Foster
- Wazim Mohammedismail
Organizations
- Army Research Office
- Indiana University
- National Institutes of Health