Adaptive Evolution of Geobacter sulfurreducens in Coculture with Pseudomonas aeruginosa
Abstract
Geobacter and Pseudomonas spp. cohabit many of the same environments, where Geobacter spp. often dominate. Both bacteria are capable of extracellular electron transfer (EET) and play important roles in biogeochemical cycling. Although they recently in 2017 were demonstrated to undergo direct interspecies electron transfer (DIET) with one another, the genetic evolution of this syntrophic interaction has not been examined. Here, we use whole-genome sequencing of the cocultures before and after adaptive evolution to determine whether genetic selection is occurring. We also probe their interaction on a temporal level and determine whether their interaction dynamics change over the course of adaptive evolution. This study brings to light the multifaceted nature of interactions between just two microorganisms within a controlled environment and will aid in improving metabolic models of microbial communities comprising these two bacteria.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 28, 2020
- Source ID
- 10.1128/mbio.02875-19
Entities
People
- Andrew Elohim Laloo
- Ashley E. Franks
- Ismael A. Vergara
- Lucie Semenec
- Philip L. Bond
- Steve Petrovski
Organizations
- Australian Research Council
- La Trobe University
- Office of Naval Research Global
- Peter MacCallum Cancer Centre
- University of Queensland