nBio Chip, a Lab-on-a-Chip Platform of Mono- and Polymicrobial Biofilms for High-Throughput Downstream Applications
Abstract
With an estimated 80% of infections being associated with a biofilm mode of growth and the ensuing recalcitrance of these biofilms with respect to conventional antibiotic treatment leading to high mortality rates, there is a dire and unmet need for the development of novel approaches to prevent, treat, and control these infections. Both bacteria and fungi are capable of forming biofilms that are inherently fragile and often polymicrobial in nature, which further complicates treatment. In this work, we showcase a nanobiofilm chip as a convenient platform for culturing several hundreds of mono- or polymicrobial biofilms and for susceptibility testing. This platform enables true ultra-high-throughput screening for antimicrobial drug discovery or diagnostics or for addressing fundamental issues in microbiology.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 28, 2017
- Source ID
- 10.1128/msphere.00247-17
Entities
People
- Anand K Ramasubramanian
- Anand Srinivasan
- Jose L Lopez-Ribot
- Kai P. Leung
- Nelson S. Torres
Organizations
- National Center for Research Resources
- National Heart, Lung, and Blood Institute
- National Institute of Allergy and Infectious Diseases
- National Institute of Dental and Craniofacial Research
- San José State University
- United States Army Materiel Command
- University of Texas at San Antonio