Modeling Group BStreptococcusand Blood-Brain Barrier Interaction by Using Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells
Abstract
Here for the first time, human iPSC-derived BMECs were used to model bacterial interaction with the BBB. Unlike models previously used to study these interactions, iPSC-derived BMECs possess robust BBB properties, such as the expression of complex tight junctions that are key components for the investigation of bacterial effects on the BBB. Here, we demonstrated that GBS interacts with the iPSC-derived BMECs and specifically disrupts these tight junctions. Thus, using this BBB model may allow researchers to uncover novel mechanisms of BBB disruption during meningitis that are inaccessible to immortalized or primary cell models that lack substantial tight junctions.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 27, 2017
- Source ID
- 10.1128/msphere.00398-17
Entities
People
- Brandon J. Kim
- Eric V Shusta
- Kelly S. Doran
- Matthew J Stebbins
- Maura A. Mcdonagh
- Olivia B. Bee
- Sean P Palecek
Organizations
- Defense Threat Reduction Agency
- National Institute of Neurological Disorders and Stroke
- University of Colorado School of Medicine
- University of Wisconsin–Madison