Protein Microarray Analysis of the Specificity and Cross-Reactivity of Influenza Virus Hemagglutinin-Specific Antibodies
Abstract
Seasonal influenza is a serious public health problem because the viral infection spreads easily from person to person and because of antigenic drift in neutralizing epitopes. Influenza vaccination is the most effective way to prevent the disease, although challenging because of the constant evolution of influenza virus subtypes. Our high-throughput protein microarrays allow for interrogation of subunit-specific IgG and IgA responses to 283 different HA proteins comprised of HA1 and HA2 domains as well as full-length HA proteins. This provides a tool that allows for novel insights into the response to exposure to influenza virus antigens. Data generated with our technology will enhance our understanding of the factors that improve the strength, breadth, and durability of vaccine-mediated immune responses and develop more effective vaccines.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 26, 2018
- Source ID
- 10.1128/msphere.00592-18
Entities
People
- Aarti Jain
- Algis Jasinskas
- D. Huw Davies
- Darrick Carter
- Donald K. Milton
- Florian Krammer
- Joshua M Obiero
- Medalyn Supnet
- Michael Grantham
- Omid Taghavian
- Philip L. Felgner
- Richard Webby
- Rie Nakajima
- Wilbur H Chen
Organizations
- Centre Pour le Développement des Vaccins-Mali
- Icahn School of Medicine at Mount Sinai
- Infectious Disease Research Institute
- Intelligence Advanced Research Projects Activity
- National Institute of Allergy and Infectious Diseases
- St. Jude Children's Research Hospital
- University of California, Irvine
- University of Maryland